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Biomicrofluidics / Volume 6 / Issue 1 / FABRICATION AND LABORATORY METHODS

Biomicrofluidics 6, 016503 (2012); http://dx.doi.org/10.1063/1.3683163 (13 pages)

A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation

Talukder Z. Jubery1, Mohammad R. Hossan1, Danny R. Bottenus2, Cornelius F. Ivory2, Wenji Dong2, and Prashanta Dutta1

1Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA
2Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164, USA

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(Received 30 November 2011; accepted 15 January 2012; published online 10 February 2012)

Recent studies show that reduction in cross-sectional area can be used to improve the concentration factor in microscale bioseparations. Due to simplicity in fabrication process, a step reduction in cross-sectional area is generally implemented in microchip to increase the concentration factor. But the sudden change in cross-sectional area can introduce significant band dispersion and distortion. This paper reports a new fabrication technique to form a gradual reduction in cross-sectional area in polymethylmethacrylate (PMMA) microchannel for both anionic and cationic isotachophoresis (ITP). The fabrication technique is based on hot embossing and surface modification assisted bonding method. Both one-dimensional and two-dimensional gradual reduction in cross-sectional area microchannels were formed on PMMA with high fidelity using proposed techniques. ITP experiments were conducted to separate and preconcentrate fluorescent proteins in these microchips. Thousand fold and ten thousand fold increase in concentrations were obtained when 10 × and 100 × gradual reduction in cross-sectional area microchannels were used for ITP.

© 2012 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. MICROCHIP FABRICATION
  3. TEST
    1. Easiness of SU-8 master fabrication
    2. Delamination of SU-8 master from substrate during hot embossing
      1. Adhesion test
      2. Thermal compatibility
  4. ISOTACHOPHORESIS
    1. Chemicals
    2. Preparation and labeling of human cTnI
    3. Electrolyte solutions
      1. Anionic ITP
      2. Cationic IT P
  5. BIOSEPARATION AND CONCENTRATION EXPERIMENTS
    1. Anionic ITP
    2. Cationic ITP experiments
  6. CONCLUDING REMARKS

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KEYWORDS, PACS, and IPC

Keywords

biochemistry, biological techniques, bioMEMS, electrophoresis, embossing, fluorescence, lab-on-a-chip, microfabrication, molecular biophysics, polymers, proteins

PACS

  • 87.80.Qk

    Biochemical separation processes

  • 87.15.Tt

    Electrophoresis

  • 07.10.Cm

    Micromechanical devices and systems

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 87.80.Ek

    Mechanical and micromechanical techniques

International Patent Classification (IPC)

  • B01D57/02

    By electrophoresis

  • B81B

    Micro-structural devices or systems, e.g. micro-mechanical devices

  • B81C1/00

    Manufacture or treatment of devices or systems in or on a substrate

  • C12

    Biochemistry; Beer; Spirits; Wine; Vinegar; Microbiology; Enzymology; Mutation or genetic engineering

  • C25

    Electrolytic or electrophoretic processes; Apparatus therefor

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3683163

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

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