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Biomicrofluidics / Volume 5 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 5, 044118 (2011); http://dx.doi.org/10.1063/1.3668243 (9 pages)

Growth propagation of yeast in linear arrays of microfluidic chambers over many generations

Li Wang1,2, Jiaji Liu2, Xin Li2, Jian Shi2, Jie Hu2, Ran Cui1, Zhi-Ling Zhang1, Dai-Wen Pang1, and Yong Chen2,3

1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, People’s Republic of China
2Ecole Normale Supérieure, CNRS-ENS-UPMC UMR 8640, 24 rue Lhomond, 75005 Paris, France
3Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8501, Japan

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(Received 30 August 2011; accepted 17 November 2011; published online 16 December 2011)

The growth of microorganisms is often confined in restricting geometries. In this work, we designed a device to study the growth propagation of budding yeast along linear arrays of microfluidic chambers. Vacuum assisted cell loading was used to seed cells of limited numbers in the up-most chambers of each linear array. Once loaded, cells grow until confluent and then overgrow, pushing some of the newborns into the neighboring downstream chamber through connection channels. Such a scenario repeats sequentially along the whole linear chamber arrays. We observed that the propagation speed of yeast population along the linear arrays was strongly channel geometry dependent. When the connection channel is narrow and long, the amount of cells delivered into the downstream chamber is small so that cells grow over several generations in the same chamber before passing into the next chamber. Consequently, a population growth of more than 50 generations could be observed along a single linear array. We also provided a mathematical model to quantitatively interpret the observed growth dynamics.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL
    1. Device preparation
    2. Cell preparation, loading, and culture
    3. Characterization and observation
  3. RESULTS AND DISCUSSION
    1. Cell growth in leading chambers
    2. Cell passage through connection channels
    3. Growth propagation by sequential chamber occupation
    4. Growth pressure induced device deformation
  4. CONCLUSION

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KEYWORDS and PACS

Keywords

biological fluid dynamics, microchannel flow, microorganisms

PACS

  • 87.85.gf

    Fluid mechanics and rheology

  • 07.10.Cm

    Micromechanical devices and systems

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3668243

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

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