BMF Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Facebook Podcast Flickr Twitter UniPHY Group iResearch App

Biomicrofluidics / Volume 5 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 5, 044111 (2011); http://dx.doi.org/10.1063/1.3664691 (10 pages)

Microfluidic concentration of bacteria by on-chip electrophoresis

Dietmar Puchberger-Enengl1,2, Susann Podszun2, Helene Heinz2, Carsten Hermann2, Paul Vulto3, and Gerald A. Urban2

1Institute of Sensor and Actuator Systems (ISAS), Vienna University of Technology, Vienna, Austria
2Laboratory for Sensors, Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universitäat Freiburg, Georges-Koehler-Allee 103, Freiburg, Germany
3Division of Analytical Biosciences, Netherlands Metabolomics Centre/LACDR, Leiden University, Leiden, Netherlands

View MapView Map

(Received 13 September 2011; accepted 31 October 2011; published online 2 December 2011)

In this contribution, we present a system for efficient preconcentration of pathogens without affecting their viability. Development of miniaturized molecular diagnostic kits requires concentration of the sample, molecule extraction, amplification, and detection. In consequence of low analyte concentrations in real-world samples, preconcentration is a critical step within this workflow. Bacteria and viruses exhibit a negative surface charge and thus can be electrophoretically captured from a continuous flow. The concept of phaseguides was applied to define gel membranes, which enable effective and reversible collection of the target species. E. coli of the strains XL1-blue and K12 were used to evaluate the performance of the device. By suppression of the electroosmotic flow both strains were captured with efficiencies of up to 99%. At a continuous flow of 15 μl/min concentration factors of 50.17 ± 2.23 and 47.36 ± 1.72 were achieved in less than 27 min for XL1-blue and K12, respectively. These results indicate that free flow electrophoresis enables efficient concentration of bacteria and the presented device can contribute to rapid analyses of swab-derived samples.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. THEORY
  3. DESIGN
  4. MATERIAL AND METHODS
    1. Finite element simulations
    2. Chip fabrication
    3. Sample preparation
    4. Experimental setup
  5. RESULTS AND DISCUSSION
  6. CONCLUSION

RELATED DATABASES

To view database links for this article, you need to log in.

KEYWORDS and PACS

Keywords

biomedical engineering, electrophoresis, lab-on-a-chip, microfluidics, microorganisms

PACS

  • 87.80.Ek

    Mechanical and micromechanical techniques

  • 87.15.Tt

    Electrophoresis

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3664691

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    C.-P. Jen and H.-H. Chang, Biomicrofluidics 5, 034101 (2011)BIOMGB000005000003034101000001.

    C. Church, J. Zhu, G. Huang, T.-R. Tzeng, and X. Xuan, Biomicrofluidics 4, 44101 (2010)BIOMGB000004000004044101000001.

    D. Hou, S. Maheshwari, and H.-C. Chang, Biomicrofluidics 1, 14106 (2007)BIOMGB000001000001014106000001.

    M. Koklu, S. Park, S. D. Pillai, and A. Beskok, Biomicrofluidics 4 (2010)BIOMGB000004000003034107000001.

    I.-F. Cheng, H.-C. Chang, D. Hou, and H.-C. Chang, Biomicrofluidics 1, 21503 (2007)BIOMGB000001000002021503000001.

    R. S. W. Thomas, P. D. Mitchell, R. O. C. Oreffo, and H. Morgan, Biomicrofluidics 4, 022806 (2010)BIOMGB000004000002022806000001


Figures (6) Multimedia (3) Tables (1)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)



Close

Google Calendar

ADVERTISEMENT

Your Recent History Recent History Help

Recently Viewed

  1. Microfluidic concentration of bacteria by on-chip electrophoresis
  2. An insulator-based dielectrophoretic microdevice for the simultaneous filtration and focusing of biological cells
  3. DNA methylation profiling in nanochannels
  4. Electrochemical biosensors for on-chip detection of oxidative stress from immune cells
  5. Continuously perfused microbubble array for 3D tumor spheroid model
Go to AIP Home
Copyright © American Institute of Physics
close