BMF Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Facebook Podcast Flickr Twitter UniPHY Group iResearch App

Biomicrofluidics / Volume 5 / Issue 3 / SPECIAL TOPIC: MICROSYSTEMS FOR MANIPULATION AND ANALYSIS OF LIVING CELLS (GUEST EDITOR: ALEXANDER REVZIN)

Biomicrofluidics 5, 032008 (2011); http://dx.doi.org/10.1063/1.3624739 (11 pages)

Electrochemical biosensors for on-chip detection of oxidative stress from immune cells

Jun Yan1, Valber A. Pedrosa2, James Enomoto1, Aleksandr L. Simonian2, and Alexander Revzin1

1Department of Biomedical Engineering, University of California, Davis, 451 East Health Sciences St. #2619, Davis, California 95616, USA
2Department of Materials Engineering, Auburn University, Auburn, Alabama 36849, USA

View MapView Map

(Received 25 January 2011; accepted 19 July 2011; published online 20 September 2011)

Seamless integration of biological components with electrochemical sensors is critical in the development of microdevices for cell analysis. The present paper describes the integration miniature Au electrodes next to immune cells (macrophages) in order to detect cell-secreted hydrogen peroxide (H2O2). Photopatterning of poly(ethylene glycol) (PEG) hydrogels was used to both immobilize horseradish peroxidase molecules onto electrodes and to define regions for cell attachment in the vicinity of sensing electrodes. Electrodes micropatterned in such a manner were enclosed inside poly(dimethylsiloxane) fluid conduits and incubated with macrophages. The cells attached onto the exposed glass regions in the vicinity of the electrodes and nowhere else on the non-fouling PEG hydrogel surface. A microfluidic device was converted into an electrochemical cell by placing flow-through Ag/AgCl reference and Pt wire counter electrodes at the outlet and inlet, respectively. This microdevice with integrated H2O2-sensing electrodes had sensitivity of 27 μA/cm2 mM with a limit of detection of 2 μM. Importantly, this microdevice allowed controllable seeding of macrophages next to electrodes, activation of these cells and on-chip monitoring of H2O2 release in real time. In the future, this biosensor platform may be utilized for monitoring of macrophage responses to pathogens or for the study of inflammatory signaling in micropatterned cell cultures.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. MATERIALS AND METHODS
    1. Materials
    2. Fabrication of Au electrode arrays
    3. Integration of enzyme-containing hydrogel microstructures with Au electrodes
    4. Fabrication of microfluidic devices for detection of cell-secreted H 2 O 2
    5. Calibration of H 2 O 2 biosensor
    6. Detecting H 2 O 2 release by activated macrophages
  3. RESULTS AND DISCUSSIONS
    1. Fabrication of hydrogel/Au electrodes
    2. Optimizing performance of H 2 O 2 -sensing electrodes
    3. Calibrating H 2 O 2 biosensors
    4. Detecting H 2 O 2 release from activated macrophages
  4. CONCLUSIONS

RELATED DATABASES

To view database links for this article, you need to log in.

KEYWORDS and PACS

Keywords

biomedical materials, bioMEMS, biosensors, cellular biophysics, electrochemical sensors, gold, hydrogels, lab-on-a-chip, microfluidics

PACS

  • 87.17.-d

    Cell processes

  • 87.85.Ox

    Biomedical instrumentation and transducers, including micro-electro-mechanical systems (MEMS)

  • 87.85.J-

    Biomaterials

  • 87.80.Kc

    Electrochemical techniques

  • 47.85.Np

    Fluidics

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3624739

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.

For access to citing articles, you need to log in.


Figures (5)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)



Close

Google Calendar

ADVERTISEMENT

Your Recent History Recent History Help

Recently Viewed

  1. Electrochemical biosensors for on-chip detection of oxidative stress from immune cells
  2. Continuously perfused microbubble array for 3D tumor spheroid model
  3. High-throughput size-based rare cell enrichment using microscale vortices
  4. Microfluidics-based devices: New tools for studying cancer and cancer stem cell migration
  5. Microfluidic devices for studying heterotypic cell-cell interactions and tissue specimen cultures under controlled microenvironments
Go to AIP Home
Copyright © American Institute of Physics
close