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Biomicrofluidics / Volume 5 / Issue 2 / SPECIAL TOPIC: MICROFLUIDICS IN CELL BIOLOGY AND TISSUE ENGINEERING (GUEST EDITOR: ALI KHADEMHOSSEINI)

Biomicrofluidics 5, 022206 (2011); http://dx.doi.org/10.1063/1.3576780 (10 pages)

High-throughput size-based rare cell enrichment using microscale vortices

Soojung Claire Hur, Albert J. Mach, and Dino Di Carlo

University of California, Los Angeles, California 90095, USA

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(Received 2 December 2010; accepted 26 January 2011; published online 29 June 2011)

Cell isolation in designated regions or from heterogeneous samples is often required for many microfluidic cell-based assays. However, current techniques have either limited throughput or are incapable of viable off-chip collection. We present an innovative approach, allowing high-throughput and label-free cell isolation and enrichment from heterogeneous solution using cell size as a biomarker. The approach utilizes the irreversible migration of particles into microscale vortices, developed in parallel expansion-contraction trapping reservoirs, as the cell isolation mechanism. We empirically determined the critical particle/cell diameter Dcrt and the operational flow rate above which trapping of cells/particles in microvortices is initiated. Using this approach we successfully separated larger cancer cells spiked in blood from the smaller blood cells with processing rates as high as 7.5×106 cells/s. Viable long-term culture was established using cells collected off-chip, suggesting that the proposed technique would be useful for clinical and research applications in which in vitro culture is often desired. The presented technology improves on current technology by enriching cells based on size without clogging mechanical filters, employing only a simple single-layered microfluidic device and processing cell solutions at the ml/min scale.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. PARTICLE TRAPPING MECHANISM
  3. MATERIAL AND METHODS
    1. Device design and fabrication
    2. Particle and cell suspension preparation
    3. Fluorescence and high-speed microscopic imaging
    4. Capturing efficiency and enrichment of larger cells using microscale-vortices
  4. RESULTS AND DISCUSSION
    1. Flow visualization and critical capturing diameter
    2. Massively parallel sized based particle/cell capturing using microscale vortices
  5. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biological fluid dynamics, biological specimen preparation, biomedical equipment, bioMEMS, blood, cancer, cellular biophysics, cellular transport, microfluidics, vortices

PACS

  • 87.85.Ox

    Biomedical instrumentation and transducers, including micro-electro-mechanical systems (MEMS)

  • 87.80.Ek

    Mechanical and micromechanical techniques

  • 87.17.Uv

    Biotechnology of cell processes

  • 87.85.gf

    Fluid mechanics and rheology

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3576780

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    D. Lim, J. Shelby, J. Kuo, and D. Chiu, Appl. Phys. Lett. 83, 1145 (2003)APPLAB000083000006001145000001.

    D. Di Carlo, J. Edd, K. Humphry, H. Stone, and M. Toner, Phys. Rev. Lett. 102, 094503 (2009).


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