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Biomicrofluidics / Volume 5 / Issue 1 / SPECIAL TOPIC: BIOLOGICAL MICROFLUIDICS IN TISSUE ENGINEERING AND REGENERATIVE MEDICINE (GUEST EDITOR: SUWAN JAYASINGHE)

Biomicrofluidics 5, 013401 (2011); http://dx.doi.org/10.1063/1.3528299 (26 pages)

Stem cells in microfluidics

Huei-Wen Wu, Chun-Che Lin, and Gwo-Bin Lee

Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan

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(Received 6 September 2010; accepted 29 November 2010; published online 30 March 2011)

Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
    1. Microfluidics
    2. Stem cells
      1. Embryonic stem cells
      2. Adult stem cells
  2. CELLS IN MICROFLUIDICS
    1. Cell culture in microfluidics
      1. 2D cell culture
      2. 3D cell culture
    2. Cell sorting and isolation in microfluidics
    3. Cell analysis in microfluidics
  3. STEM CELL TYPES AND APPLICATIONS IN MICROFLUIDICS
    1. Embryonic stem cells
      1. Size-controlled microwells
      2. Membrane fabrication
      3. Feeder-layer coating
      4. Arrayed microreactors and microwells
      5. Physical factors
      6. Chemical factors
    2. Mesenchymal stem cells
      1. Microculture and differentiation environment
      2. Chemical and physical factors
      3. Patterning structures
      4. MSC coculture
      5. MSC separation and transfection
    3. Hematopoietic stem cells
      1. Purification and separation
      2. Microwells and microcavities
      3. Microelectrophoresis assay and digital RT-PCR assay
    4. Neural stem cells
      1. Signaling analysis
      2. NSC sorting and gene expression profiling
    5. Integrated microsystems for stem cell applications
  4. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biological techniques, bioMEMS, cellular biophysics, microfluidics

PACS

  • 87.80.Ek

    Mechanical and micromechanical techniques

  • 87.17.-d

    Cell processes

  • 87.16.-b

    Subcellular structure and processes

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 47.85.Np

    Fluidics

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3528299

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    F. Xiao, T. H. Wu, and P. Y. Chiou, Appl. Phys. Lett. 97, 031112 (2010)APPLAB000097000003031112000001.

    J. W. van Honschoten, J. W. Berenschot, T. Ondarcuhu, R. G. P. Sanders, J. Sundaram, M. Elwenspoek, and N. R. Tas, Appl. Phys. Lett. 97, 014103 (2010)APPLAB000097000001014103000001.

    W. Dai, Y. Z. Zheng, K. Q. Luo, and H. K. Wu, Biomicrofluidics 4, 024101 (2010)BIOMGB000004000002024101000001.

    J. B. Edel and A. J. Demello, Appl. Phys. Lett. 90, 053904 (2007)APPLAB000090000005053904000001.

    T. E. Angelini, E. Hannezo, X. Trepat, J. J. Fredberg, and D. A. Weitz, Phys. Rev. Lett. 104, 168104 (2010).

    H. W. Wu, R. C. Hsu, C. C. Lin, S. M. Hwang, and G. B. Lee, Biomicrofluidics 4, 024112 (2010)BIOMGB000004000002024112000001.


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