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Biomicrofluidics / Volume 4 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 4, 044111 (2010); http://dx.doi.org/10.1063/1.3523057 (9 pages)

Passive optical separation and enrichment of cells by size difference

Siew-Kit Hoi1, Vuong Hoang Kim2, Nguyen Manh Huy2, Chorng-Haur Sow1, Yueh-Sheng Ow1, and Andrew A. Bettiol1

1Department of Physics, National University of Singapore, Blk S12, 2 Science Drive 3, 117542 Singapore, Singapore
2NUS High School of Mathematics and Science, 20 Clementi Avenue 1, 129957 Singapore, Singapore

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(Received 3 September 2010; accepted 10 November 2010; published online 6 December 2010)

A size-selective cell sorting microfluidic device that utilizes optical force is developed. The device consists of a three-dimensional polydimethylsiloxane microstructure comprised of two crossed microchannels in a three-dimensional configuration. A line shaped focused laser beam is used for automatic size-selective cell sorting in a continuous flow environment. As yeast cells in an aqueous medium are fed continuously into a lower channel, the line shaped focused laser beam is applied (perpendicular to the direction of flow) at the junction of the two crossed channels. The scattering force of the laser beam was employed to push cells matching specific criteria upward from one channel to another. The force depends on the size of the cells, the laser power, and the fluid flow speed. The variation in size of yeast cells causes them to follow different routes at the intersection. For flow speeds below 30 μm/s, all yeast cells larger than 3 μm were removed from the main stream. As a result, a high purity sample of small cells can be collected at the outlet of bottom channel.

© 2010 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL
    1. Fabrication of microfluidic chips
    2. Experimental procedures
  3. RESULTS AND DISCUSSION
  4. CONCLUSION

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KEYWORDS and PACS

Keywords

biological techniques, bioMEMS, cellular biophysics, laser beam applications, microchannel flow, microorganisms, polymers

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3523057

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
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