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Biomicrofluidics / Volume 4 / Issue 3 / REGULAR ARTICLES

Biomicrofluidics 4, 034109 (2010); http://dx.doi.org/10.1063/1.3481777 (11 pages)

Contraction and extension of Vorticella and its mechanical characterization under flow loading

Moeto Nagai1, Hiroshi Asai2, and Hiroyuki Fujita1

1Center for International Research on Micro Mechatronics, Institute of Industrial Science, The University of Tokyo, Tokyo 153-5805, Japan
2Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan

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(Received 10 June 2010; accepted 3 August 2010; published online 26 August 2010)

We have studied the contraction and extension of Vorticella convallaria and its mechanical properties with a microfluidic loading system. Cells of V. convallaria were injected to a microfluidic channel (500 μm in width and 100 μm in height) and loaded by flow up to ∼ 350 mm s−1. The flow produced a drag force on the order of nanonewton on a typical vorticellid cell body. We gradually increased the loading force on the same V. convallaria specimen and examined its mechanical property and stalk motion of V. convallaria. With greater drag forces, the contraction distance linearly decreased; the contracted length was close to around 90% of the stretched length. We estimated the drag force on Vorticella in the channel by calculating the force on a sphere in a linear shear flow.

© 2010 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. METHOD
    1. Device fabrication
    2. Cell preparation
    3. Flow rate control and cell loading
    4. Data acquisition
  3. RESULTS AND DISCUSSIONS
    1. Adherence and alignment of V. convallaria in a microfluidic channel
    2. Contraction, extension, and cell stretching of V. convallaria
    3. Deformation and breakage of V. convallaria
    4. Decrease in stalk contraction
    5. Ambient flow around V. convallaria in the microfluidic channel
    6. Estimation of drag force on V. convallaria
    7. Changes in stalk contraction due to drag force
  4. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biological fluid dynamics, bioMEMS, cellular biophysics, drag, microchannel flow, microorganisms, shear flow

PACS

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 47.85.Np

    Fluidics

  • 87.16.-b

    Subcellular structure and processes

  • 47.63.-b

    Biological fluid dynamics

  • 87.85.gf

    Fluid mechanics and rheology

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3481777

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    M. Nagai, M. Oishi, M. Oshima, H. Asai, and H. Fujita, Biomicrofluidics 3, 014105 (2009)BIOMGB000003000001014105000001.

    W. Dai, Y. Zheng, K. Q. Luo, and H. Wu, Biomicrofluidics 4, 024101 (2010)BIOMGB000004000002024101000001.

    S. A. Vanapalli, M. H. G. Duits, and F. Mugele, Biomicrofluidics 3, 012006 (2009)BIOMGB000003000001012006000001.

    A. D. van der Meer, A. A. Poot, J. Feijen, and I. Vermes, Biomicrofluidics 4, 011103 (2010)BIOMGB000004000001011103000001.

    S. Chiavaroli, D. Newport, and B. Woulfe, Biomicrofluidics 4, 024110 (2010)BIOMGB000004000002024110000001.

    L. Zeng, F. Najjar, S. Balachandar, and P. Fischer, Phys. Fluids 21, 033302 (2009)PHFLE6000021000003033302000001.


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