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Biomicrofluidics / Volume 3 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 3, 044109 (2009); http://dx.doi.org/10.1063/1.3267098 (10 pages)

Electrokinetic focusing and filtration of cells in a serpentine microchannel

Christopher Church1, Junjie Zhu1, Gaoyan Wang2, Tzuen-Rong J. Tzeng2, and Xiangchun Xuan1

1Department of Mechanical Engineering, Clemson University, Clemson, South Carolina 29634-0921, USA
2Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634-0314, USA

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(Received 2 October 2009; accepted 2 November 2009; published online 24 November 2009)

Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased ac electric fields. The concurrent pumping and focusing of yeast cells arise from the dc electrokinetic transport and the turn-induced ac/dc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. coli cells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.

© 2009 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENT
    1. Microchannel fabrication
    2. Cell culture
    3. Experimental technique
  3. THEORY
    1. Operating mechanism
    2. Numerical modeling
  4. RESULTS AND DISCUSSIONS
    1. Electric field effects on yeast cell focusing
    2. Concentration effects on yeast cell focusing
    3. Electrokinetic filtration of E. coli cells from yeast cells
    4. Joule heating and cell viability test
  5. CONCLUSION

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KEYWORDS and PACS

Keywords

biochemistry, bioelectric phenomena, biological effects of fields, cellular effects of radiation, electrokinetic effects, electrophoresis, microchannel flow

PACS

  • 87.50.ch

    Electrophoresis/dielectrophoresis and other mechanical effects

  • 87.16.-b

    Subcellular structure and processes

  • 82.45.Tv

    Bioelectrochemistry

  • 07.10.Cm

    Micromechanical devices and systems

  • 87.15.Tt

    Electrophoresis

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3267098

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    T. A. Lin, A. E. Hosoi, and D. J. Ehrlich, Biomicrofluidics 3, 014101 (2009)BIOMGB000003000001014101000001.

    N. Lewpiriyawong, C. Yang, and Y. C. Lam, Biomicrofluidics 2, 034105 (2008)BIOMGB000002000003034105000001.

    I. F. Cheng, H. C. Chang, D. Hou, and H. C. Chang, Biomicrofluids 1, 021503 (2007)BIOMGB000001000002021503000001.

    J. Seo, M. H. Lean, and A. Kole, Appl. Phys. Lett. 91, 033901 (2007)APPLAB000091000003033901000001.

    K. Hyoung Kang, X. Xuan, Y. Kang, and D. Li, J. Appl. Phys. 99, 064702 (2006)JAPIAU000099000006064702000001.


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