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Biomicrofluidics / Volume 3 / Issue 1 / REGULAR ARTICLES

Biomicrofluidics 3, 014103 (2009); http://dx.doi.org/10.1063/1.3086600 (10 pages)

Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force

Hyundoo Hwang, Do-Hyun Lee, Wonjae Choi, and Je-Kyun Park

Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea

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(Received 21 November 2008; accepted 30 January 2009; published online 17 February 2009)

We present a method to discriminate normal oocytes in an optoelectrofluidic platform based on the optically induced positive dielectrophoresis (DEP) for in vitro fertilization. By combining the gravity with a pulling-up DEP force that is induced by dynamic image projected from a liquid crystal display, the discrimination performance could be enhanced due to the reduction in friction force acting on the oocytes that are relatively large and heavy cells being affected by the gravity field. The voltage condition of 10 V bias at 1 MHz was applied for moving normal oocytes. The increased difference of moving velocity between normal and starved abnormal oocytes allows us to discriminate the normal ones spontaneously under the moving image pattern. This approach can be useful to develop an automatic and interactive selection tool of fertilizable oocytes.

© 2009 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. PRINCIPLES AND THEORY
    1. DEP characteristics of oocyte
    2. Two manipulation schemes of optoelectrofluidic platform
  3. EXPERIMENTAL
    1. Preparation of porcine oocytes
    2. Fabrication of optoelectrofluidic device
    3. Experimental setup of optoelectrofluidic platform
  4. RESULTS AND DISCUSSION
  5. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biological techniques, cellular biophysics, electrophoresis, fluidics, liquid crystal displays, optoelectronic devices, radiation pressure

PACS

  • 87.50.ch

    Electrophoresis/dielectrophoresis and other mechanical effects

  • 87.80.Cc

    Optical trapping

  • 85.60.-q

    Optoelectronic devices

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3086600

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    H. Hwang, Y. Oh, J. -J. Kim, W. Choi, J. -K. Park, S. -H. Kim, and J. Jang, Appl. Phys. Lett. 92, 024108 (2008)APPLAB000092000002024108000001.


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