BMF Nameplate
  • Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Facebook Podcast Flickr Twitter UniPHY Group iResearch App

Biomicrofluidics / Volume 2 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 2, 044103 (2008); http://dx.doi.org/10.1063/1.3037326 (10 pages)

Long-range and superfast trapping of DNA molecules in an ac electrokinetic funnel

Jiong-Rong Du, Yi-Je Juang, Jie-Tang Wu, and Hsien-Hung Wei

Department of Chemical Engineering and Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan

View MapView Map

(Received 9 September 2008; accepted 3 November 2008; published online 5 December 2008)

In this work we report a microfluidic platform capable of trapping and concentrating a trace amount of DNA molecules efficiently. Our strategy invokes nonlinear electro-osmotic flow induced by charge polarization under high-frequency ac fields. With the asymmetric quadrupole electrode design, a unique converging flow structure can be created for generating focusing effects on DNA molecules. This focusing in turn transforms into a robust funnel that can collect DNA molecules distantly from the bulk and pack them into a compact cone with the aid of short-range dipole-induced self-attraction and dielectrophoresis. Our results reveal that not only can DNA molecules be concentrated within just a few seconds, but also they can be focused into threads of 1 mm in length, demonstrating the superfast and long-range trapping capability of this funnel. In addition, pico M DNA solutions can be concentrated with several decades of enhancement without any continuous feeding. Alternating concentration and release of DNA molecules is also illustrated, which has potentials in concentrating and transporting biomolecules in a continuous fashion using microdevices.

© 2008 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL SECTION
  3. RESULTS AND DISCUSSION
    1. Hydrodynamic focusing by asymmetric ac electro-osmotic flow
    2. Dipole-induced trapping of DNA molecules expedited by ACEO focusing
    3. Rapid concentration of dilute DNA molecules
  4. CONCLUDING REMARKS

RELATED DATABASES

To view database links for this article, you need to log in.

KEYWORDS and PACS

Keywords

biological techniques, DNA, electrokinetic effects, electrophoresis, microfluidics, osmosis

PACS

  • 87.80.Ek

    Mechanical and micromechanical techniques

  • 47.61.Fg

    Flows in micro-electromechanical systems (MEMS) and nano-electromechanical systems (NEMS)

  • 47.85.Np

    Fluidics

  • 87.15.Tt

    Electrophoresis

  • 87.50.ch

    Electrophoresis/dielectrophoresis and other mechanical effects

  • 47.57.jd

    Electrokinetic effects

  • 82.39.Wj

    Ion exchange, dialysis, osmosis, electro-osmosis, membrane processes

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3037326

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    N. G. Green, A. Ramos, A. Gonzalez, H. Morgan, and A. Castellanos, Phys. Rev. E 61, 4011 (2000)
    A. Gonzalez, A. Ramos, N. G. Green, A. Castellanos, and H. Morgan, ibid. 61, 4019 (2000).

    J. T. Wu, J. R. Du, Y. J. Juang, and H.-H. Wei, Appl. Phys. Lett. 90, 134103 (2007)APPLAB000090000013134103000001.

    D. Lastochkin, R. Zhou, P. Wang, Y. Ben, and H.-C. Chang, J. Appl. Phys. 96, 1730 (2004)JAPIAU000096000003001730000001.

    S. Fraden, A. J. Hurd, and R. B. Meyer, Phys. Rev. Lett. 63, 2373 (1989).


For access to citing articles, you need to log in.


Figures (7)

Access to article objects (figures, tables, multimedia) requires a subscription; log in to view available files.
(Access to supplementary files, where available, is free for this journal.)



Close

Google Calendar

ADVERTISEMENT

Your Recent History Recent History Help

Recently Viewed

  1. Long-range and superfast trapping of DNA molecules in an ac electrokinetic funnel
  2. Dielectrophoretic manipulation of particles in a modified microfluidic H filter with multi-insulating blocks
  3. Design and integration of an all-in-one biomicrofluidic chip
  4. Size-dependent trajectories of DNA macromolecules due to insulative dielectrophoresis in submicrometer-deep fluidic channels
  5. Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking
Go to AIP Home
Copyright © American Institute of Physics
close