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Biomicrofluidics / Volume 2 / Issue 2 / REGULAR ARTICLES

Biomicrofluidics 2, 024103 (2008); http://dx.doi.org/10.1063/1.2930817 (14 pages)

Size-dependent trajectories of DNA macromolecules due to insulative dielectrophoresis in submicrometer-deep fluidic channels

Gea O. F. Parikesit1,2, Anton P. Markesteijn2, Oana M. Piciu3, Andre Bossche3, Jerry Westerweel2, Ian T. Young1, and Yuval Garini4

1Quantitative Imaging Group, Delft University of Technology, Lorentzweg 1, 2628 CJ, Delft, The Netherlands
2Laboratory for Aero and Hydrodynamics, Delft University of Technology, Leeghwaterstraat 21, 2628 CA, Delft, The Netherlands
3Electronic Instrumentation Laboratory, Delft University of Technology, Mekelweg 4, 2628 CD, Delft, The Netherlands
4Physics Department and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan 52900, Israel

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(Received 3 March 2008; accepted 22 April 2008; published online 6 May 2008)

In this paper, we demonstrate for the first time that insulative dielectrophoresis can induce size-dependent trajectories of DNA macromolecules. We experimentally use λ (48.5 kbp) and T4GT7 (165.6 kbp) DNA molecules flowing continuously around a sharp corner inside fluidic channels with a depth of 0.4 μm. Numerical simulation of the electrokinetic force distribution inside the channels is in qualitative agreement with our experimentally observed trajectories. We discuss a possible physical mechanism for the DNA polarization and dielectrophoresis inside confining channels, based on the observed dielectrophoresis responses due to different DNA sizes and various electric fields applied between the inlet and the outlet. The proposed physical mechanism indicates that further extensive investigations, both theoretically and experimentally, would be very useful to better elucidate the forces involved at DNA dielectrophoresis. When applied for size-based sorting of DNA molecules, our sorting method offers two major advantages compared to earlier attempts with insulative dielectrophoresis: Its continuous operation allows for high-throughput analysis, and it only requires electric field strengths as low as ∼ 10 V/cm.

© 2008 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. METHODS
  3. RESULTS AND DISCUSSIONS
  4. PHYSICAL MECHANISM OF CONFINED DNA DIELECTROPHORESIS
  5. CONCLUSION

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KEYWORDS and PACS

Keywords

biological techniques, bioMEMS, channel flow, DNA, electrokinetic effects, electrophoresis, microfluidics, molecular biophysics

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2930817

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    K. H. Kang, X. Xuan, Y. Kang, and D. Li, J. Appl. Phys. 99, 064702 (2006)JAPIAU000099000006064702000001.

    J. -L. Viovy, Rev. Mod. Phys. 72, 813 (2000).

    W. Reisner, K. J. Morton, R. Riehn, Y. M. Wang, Z. Yu, M. Rosen, J. C. Sturm, S. Y. Chou, E. Frey, and R. H. Austin, Phys. Rev. Lett. 94, 196101 (2005).


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