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Biomicrofluidics / Volume 1 / Issue 4 / REGULAR ARTICLES

Biomicrofluidics 1, 044102 (2007); http://dx.doi.org/10.1063/1.2818767 (5 pages)

Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking

Jason E. Gordon, Zachary Gagnon, and Hsueh-Chia Chang

Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA

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(Received 27 July 2007; accepted 5 November 2007; published online 27 November 2007)

We report an interesting buffer electric relaxation time tuning technique, coupled with a glutaraldehyde cross-linking cell fixation reaction, which allows for sensitive dielectrophoretic analysis and discrimination of bovine red blood cell (bRBC) starvation age. The buffer composition is selected such that two easily accessible dielectrophoretic crossover frequencies (cof) exist. Low concentration glutaraldehyde fixation was observed to produce a threefold decrease in the higher cof with a comparable increase in the lower cof also witnessed. More importantly, increased glutaraldehyde fixation concentration significantly increased the higher cof by a factor found to be sensitive to the bRBC starvation age.

© 2007 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. MATERIALS AND METHODS
  3. RESULTS AND DISCUSSION
  4. ACKNOWLEDGMENTS

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KEYWORDS and PACS

Keywords

biochemistry, bioelectric phenomena, biomembranes, cellular biophysics, electrophoresis, microfluidics, organic compounds

PACS

  • 87.50.C-

    Static and low-frequency electric and magnetic fields effects

  • 87.16.D-

    Membranes, bilayers, and vesicles

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2818767

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

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