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Biomicrofluidics / Volume 1 / Issue 2 / SPECIAL TOPIC: PAPERS FROM THE 2006 ANNUAL MEETING OF THE AMERICAN ELECTROPHORESIS SOCIETY, SAN FRANCISCO, CALIFORNIA, 2006

Biomicrofluidics 1, 021503 (2007); http://dx.doi.org/10.1063/1.2723669 (15 pages)

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

I-Fang Cheng1, Hsien-Chang Chang1, Diana Hou2, and Hsueh-Chia Chang2

1Institute of Nanotechnology and Microsystem Engineering, Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China
2Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556

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(Received 21 December 2006; accepted 1 March 2007; published online 10 May 2007)

Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3 μL/min. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 μl of a heterogeneous colony of 107 colony forming units /ml was processed in a single pass within 30 min.

© 2007 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. ELECTRODE DESIGN AND LAYOUT
    1. DEP gating for sorting and trapping
    2. Chip layout
  3. MATERIALS AND METHODS
    1. Micro-electrode fabrication
    2. Micro-channel fabrication
  4. EXPERIMENTAL SETUP
    1. Sample preparation
    2. Methods
  5. RESULTS AND DISCUSSION
    1. DEP properties/filtering
    2. Focusing
    3. Particle sorting
    4. Particle trapping
    5. Detection
  6. CONCLUSIONS

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KEYWORDS and PACS

Keywords

patient diagnosis, bioMEMS, electrophoresis, bioelectric phenomena, microfluidics, microelectrodes, surface enhanced Raman scattering, optical sensors, microsensors, bio-optics

PACS

  • 87.80.-y

    Biophysical techniques (research methods)

  • 87.19.R-

    Mechanical and electrical properties of tissues and organs

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 82.45.Tv

    Bioelectrochemistry

  • 47.85.Np

    Fluidics

  • 07.07.Df

    Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2723669

PUBLICATION DATA

ISSN

1932-1058 (print)  
1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    D. J. Bennett, B. Khusid, C. D. James, P. C. Balambos, M. Okandan, D. Jacqmin, and A. Acrivos, Appl. Phys. Lett. 83, 4866 (2003)APPLAB000083000023004866000001.

    S. K. Thamida and H.-C. Chang, Phys. Fluids 14, 4315 (2002)PHFLE6000014000012004315000001.


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