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Biomicrofluidics / Volume 1 / Issue 1 / REGULAR ARTICLES

Biomicrofluidics 1, 014106 (2007); http://dx.doi.org/10.1063/1.2710191 (13 pages)

Rapid bioparticle concentration and detection by combining a discharge driven vortex with surface enhanced Raman scattering

Diana Hou, Siddharth Maheshwari, and Hsueh-Chia Chang

Department of Chemical and Biomolecular Engineering, Center for Microfluidics and Medical Diagnostics, University of Notre Dame, Notre Dame, Indiana 46556

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(Received 10 November 2006; accepted 28 January 2007; published online 16 February 2007)

Rapid concentration and detection of bacteria in integrated chips and microfluidic devices is needed for the advancement of lab-on-a-chip devices because current detection methods require high concentrations of bacteria which render them impractical. We present a new chip-scale rapid bacteria concentration technique combined with surface-enhanced Raman scattering (SERS) to enhance the detection of low bacteria count samples. This concentration technique relies on convection by a long-range converging vortex to concentrate the bacteria into a packed mound of 200 μm in diameter within 15 min. Concentration of bioparticle samples as low as 104 colony forming units (CFU)/ml are presented using batch volumes as large as 150 μl. Mixtures of silver nanoparticles with Saccharomyces cerevisiae, Escherichia coli F-amp, and Bacillus subtilis produce distinct and noticeably different Raman spectra, illustrating that this technique can be used as a detection and identification tool.

© 2007 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. THEORY
    1. Ionic wind generation
    2. Flow field
    3. Spiral electrode
  3. EXPERIMENTAL SECTION
    1. Materials
    2. Methods
  4. RESULTS AND DISCUSSION
    1. Latex particles
    2. S. cerevisiae concentration and detection
    3. E. coli concentration and detection
    4. B. subtilis concentration and detection
    5. Spiral electrode
  5. CONCLUSION

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KEYWORDS and PACS

Keywords

microorganisms, microfluidics, lab-on-a-chip, bioMEMS, Raman spectra, vortices, convection, silver, nanoparticles, microsensors, optical sensors, patient diagnosis

PACS

  • 87.80.-y

    Biophysical techniques (research methods)

  • 85.85.+j

    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

  • 47.85.Np

    Fluidics

  • 47.61.Fg

    Flows in micro-electromechanical systems (MEMS) and nano-electromechanical systems (NEMS)

  • 47.32.cb

    Vortex interactions

  • 07.10.Cm

    Micromechanical devices and systems

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.2710191

PUBLICATION DATA

ISSN

1932-1058 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    L. Yeo, D. Hou, S. Maheshwari, and H.-C. Chang, Appl. Phys. Lett. 88, 233512 (2006)APPLAB000088000023233512000001.

    L. Yeo, J. R. Friend, and D. R. Arifin, Appl. Phys. Lett. 89, 103516 (2006)APPLAB000089000010103516000001;, D. R. Arifin, L. Y. Yeo, and J. R. Friend, Biomicrofluidics 1, 014103 (2007)BIOMGB000001000001014103000001.


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