Welcome to the Biomicrofluidics Video Gallery, a collection of videos brought together from the peer-reviewed articles that have appeared in BMF since the publication of Volume 1 in 2007. Each contributing author has written an additional short description to each entry to clarify each video's content. Each thumbnail image is linked directly to the movie file (.MOV, .MPG, .WMV, etc.) on AIP's content server, Scitation, and a link to the BMF article, in which each video originally appeared, is also made available.

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Previously conducted video contest entries and archived material are not part of this gallery.

Passive optical separation and enrichment of cells by size difference.

Video of yeast cells passing through the intersection. Small and large yeast cells were initially flowing in the bottom channel. When they encounter the line laser, the large yeast cell are stopped and pushed to the upper channel while the small yeast cell passed straight through. As a result, the large cells and small cells were successfully separated.

from Passive optical separation and enrichment of cells by size difference, Siew-Kit Hoi, Vuong Hoang Kim, Nguyen Manh Huy, Chorng-Haur Sow, Yueh-Sheng Ow, and Andrew A. Bettiol, Biomicrofluidics 4, 044111 (2010).

Behavior of a train of droplets in a fluidic network with hydrodynamic traps.

This study investigates the dynamics of a train of droplets in a fluidic network with hydrodynamic traps. The movie shows uniform doublet trapping, where the conditions were R_l/R_u = 1.56, Q_w/Q_o = 0.5, and Q_o = 10µl/h.

from Behavior of a train of droplets in a fluidic network with hydrodynamic traps, Swastika S. Bithi and Siva A. Vanapalli, Biomicrofluidics 4, 044110 (2010).

Contraction and extension of Vorticella and its mechanical characterization under flow loading.

The flow effect on contraction, extension, and shape of V. convallaria. Zooids were stretched by drag forces, and the lengths of zooids increased. The bell-shaped zooid becomes spherical when it contracted as shown in the videos. The length changes of the zooid during contraction and extension gradually increased to ~20–40 µm for flow rates between 100 and 1100 µl min^-1. The zooids completed their contractions within the time frame of 30 or 40 ms.

from Contraction and extension of Vorticella and its mechanical characterization under flow loading, Moeto Nagai, Hiroshi Asai, and Hiroyuki Fujita, Biomicrofluidics 4, 034109 (2010).

Contraction and extension of Vorticella and its mechanical characterization under flow loading.

The flow effect on contraction, extension, and shape of V. convallaria. Zooids were stretched by drag forces, and the lengths of zooids increased. The bell-shaped zooid becomes spherical when it contracted as shown in the videos. The length changes of the zooid during contraction and extension gradually increased to ~20–40 µm for flow rates between 100 and 1100 µl min^-1. The zooids completed their contractions within the time frame of 30 or 40 ms.

from Contraction and extension of Vorticella and its mechanical characterization under flow loading, Moeto Nagai, Hiroshi Asai, and Hiroyuki Fujita, Biomicrofluidics 4, 034109 (2010).

A method for reducing pressure-induced deformation in silicone microfluidics.

Fabrication and Laboratory Methods video showing fabrication of a glass-PDMS-glass device from a 3 inch silicon wafer master.

from A method for reducing pressure-induced deformation in silicone microfluidics, David W. Inglis, Biomicrofluidics 4, 026504 (2010).

Electric charge-mediated coalescence of water droplets for biochemical microreactorsle.

Two charged droplets of phenolphthalein solution and NaOH solution are merged by the attractive electrostatic force.

from Electric charge-mediated coalescence of water droplets for biochemical microreactorsle, Yong-Mi Jung (정용미) and In Seok Kang (강인석), Biomicrofluidics 4, 024104 (2010).

Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array.

The time lapse observation of the collected fusants was made by loading the PDMS chip to a microscope equipped with on-stage incubator. Synchronized cell division observed on chips with smaller micro-orifice (diameter ~ 2µm). Here we see actual time lapse images of the synchronized cell division.

from Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array, Murat Gel, Yuji Kimura, Osamu Kurosawa, Hidehiro Oana, Hidetoshi Kotera, and Masao Washizu , Biomicrofluidics 4, 022808 (2010).

Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array.

The time lapse observation of the collected fusants was made by loading the PDMS chip to a microscope equipped with on-stage incubator. Here, five cell pairs are shown fused on the chip at the beginning of the incubation period, the collected fusants at the beginning of the time lapse imaging, and the actual images during cell division.

from Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array, Murat Gel, Yuji Kimura, Osamu Kurosawa, Hidehiro Oana, Hidetoshi Kotera, and Masao Washizu , Biomicrofluidics 4, 022808 (2010).

An article from the journal's newest section: "Fabrication and Laboratory Methods." The embedded multimedia and associated text go step by step through a specific method or procedure.[http://dx.doi.org/10.1063/1.3259624.1] A detailed protocol is provided here for using PDMS in the fabrication of microfluidic devices to aid those interested in using the material in their work, with information on the many potential ways the material may be used for novel devices.

from Fabrication of microfluidic devices using polydimethylsiloxane, James Frienda and Leslie Yeo , Biomicrofluidics 4, 026502 (2010).

from Using laser Doppler vibrometry to measure capillary surface waves on fluid-fluid interfaces, James Frienda and Leslie Yeo, Biomicrofluidics 4, 026501 (2010).

Onset of channeling during DNA electrophoresis in a sparse ordered post array. Even at a shorter distance of 10 mm, the videomicroscopy data presented definitively identify the peaks in the electropherogram. The first wave of DNA to enter the viewing window clearly consists of a large number of small, freely moving DNA, corresponding to pUC19. In contrast, the latter images feature a lower number of bright molecules, corresponding to λ-DNA. These large DNAs also occasionally undergo rope-over-pulley collisions at 20 V/cm. [http://dx.doi.org/10.1063/].

from Onset of channeling during DNA electrophoresis in a sparse ordered post array, Jia Ou, Samuel J. Carpenter, and Kevin D. Dorfman, Biomicrofluidics 4, 013203 (2010).

The normal mobility behavior of the worms without treatment of neurotoxin MPP+. This movie showed the mobility behavior of the individual worms in the absence of MPP+. The untreated worms exhibited free movements with more often sine wave-shape and C-shape movement state within the microchannels.

from A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans, Hui Ma, Lei Jiang, Weiwei Shi, Jianhua Qin, and Bingcheng Lin, Biomicrofluidics 3, 044114 (2009).

The slight mobility defects of the worms treated with neurotoxin at lower concentration. The worms exhibited slow stroke frequency without obvious change of their movement state within the microchannels after treated with 0.5mM MPP+.

from A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans, Hui Ma, Lei Jiang, Weiwei Shi, Jianhua Qin, and Bingcheng Lin, Biomicrofluidics 3, 044114 (2009).

The obvious mobility defects of the worms treated with neurotoxin at higher concentration. The worms exhibited the slow stroke frequency with obvious coiled or titanic state within the microchannels after treated with 1.0mM MPP+.

from A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans, Hui Ma, Lei Jiang, Weiwei Shi, Jianhua Qin, and Bingcheng Lin, Biomicrofluidics 3, 044114 (2009).

The movies from this article show the patterns of bubbles in a liquid stream generated by a microfluidic device made of two concentric tapered capillary micropipettes. The inner channel is pumped with air and the outer channel is pumped with 1% alginate solution containing 1% Pluronic F127 surfactant.

The first movie shows the pattern changes by reducing the outer liquid flow rate at a constant air pressure. The notation shows pressure (psi)/flow rate (µl/min). (a) shows bubbles travel through a liquid jet with a diameter the same as the outer orifice. The liquid jet becomes thinner liquid thread connecting between bubbles after a certain distance. (b) shows that the liquid thread between bubbles become thinner and shorter. (c) shows bidisperse bubbles are encapsulated in monodisperse liquid droplets. (d) shows liquid droplets alternating between one bubble encapsulated or no bubbles (burst). (e) shows liquid droplets containing no bubbles.

from Fabricating scaffolds by microfluidics, Kuo-yuan Chung, Narayan Chandra Mishra, Chen-chi Wang, Feng-hui Lin, and Keng-hui Lin, Biomicrofluidics 3, 022403 (2009).

The movies from this article show the patterns of bubbles in a liquid stream generated by a microfluidic device made of two concentric tapered capillary micropipettes. The inner channel is pumped with air and the outer channel is pumped with 1% alginate solution containing 1% Pluronic F127 surfactant.

This movie shows more interesting patterns at various gas pressure and liquid flow rate. (a) shows a long train of bubbles in a liquid stream with diameter similar to the diameter of bubbles. (b) shows a shorter train of bubbles in a liquid stream. (c) shows monodisperse bubbles encapsulated in monodisperse liquid droplets. (d) shows bidisperse bubbles encapsulated in monodisperse liquid droplets. (e) shows unstable bubbles encapsulated in liquid droplets.

from Fabricating scaffolds by microfluidics, Kuo-yuan Chung, Narayan Chandra Mishra, Chen-chi Wang, Feng-hui Lin, and Keng-hui Lin, Biomicrofluidics 3, 022403 (2009).

Experimental condition: E = 1.5 kV/cm, V = 2 ml, n = 10 cSt

from A novel actuation method of transporting droplets by using electrical charging of droplet in a dielectric fluid, Yong-Mi Jung (정용미) and In Seok Kang (강인석) , Biomicrofluidics 3, 022402 (2009).

from Modeling the coalescence of sessile droplets, M. Sellier and E. Trelluyer, Biomicrofluidics 3, 022412 (2009).

from Understanding electrokinetics at the nanoscale: A perspective, Hsueh-Chia Chang and Gilad Yossifon, Biomicrofluidics 3, 012001 (2009).

from Understanding electrokinetics at the nanoscale: A perspective, Hsueh-Chia Chang and Gilad Yossifon, Biomicrofluidics 3, 012001 (2009).

from Understanding electrokinetics at the nanoscale: A perspective, Hsueh-Chia Chang and Gilad Yossifon, Biomicrofluidics 3, 012001 (2009).

from Understanding electrokinetics at the nanoscale: A perspective, Hsueh-Chia Chang and Gilad Yossifon, Biomicrofluidics 3, 012001 (2009).

from Investigation of microflow reversal by ac electrokinetics in orthogonal electrodes for micropump design, Kai Yang and Jie Wu, Biomicrofluidics 2, 024101 (2008).

from Investigation of microflow reversal by ac electrokinetics in orthogonal electrodes for micropump design, Kai Yang and Jie Wu, Biomicrofluidics 2, 024101 (2008).

from Investigation of microflow reversal by ac electrokinetics in orthogonal electrodes for micropump design, Kai Yang and Jie Wu, Biomicrofluidics 2, 024101 (2008).

from A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier, Ji-Yen Cheng, Meng-Hua Yen, Ching-Te Kuo, and Tai-Horng Young, Biomicrofluidics 2, 024105 (2008).

from A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier, Ji-Yen Cheng, Meng-Hua Yen, Ching-Te Kuo, and Tai-Horng Young, Biomicrofluidics 2, 024105 (2008).

from A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier, Ji-Yen Cheng, Meng-Hua Yen, Ching-Te Kuo, and Tai-Horng Young, Biomicrofluidics 2, 024105 (2008).

from Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking, J.E. Gordon, Z. Gagnon, and H.-C. Chang, Biomicrofluidics 1, 044102 (2007).

from An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting, I.F. Cheng, H.C. Chang, D. Hou, and H.-C. Chang, Biomicrofluidics 1, 021503 (2007).

from An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting, I.F. Cheng, H.C. Chang, D. Hou, and H.-C. Chang, Biomicrofluidics 1, 021503 (2007).

from An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting, I.F. Cheng, H.C. Chang, D. Hou, and H.-C. Chang, Biomicrofluidics 1, 021503 (2007).